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Heparin Sulfate Arrays to Probe Binding Protein Specificity

Although hundreds of heparan sulfate binding proteins have been identified, and implicated in a myriad of physiological and pathological processes, very little information is known about ligand requirements for binding and mediating biological activities by these proteins. This difficulty results from a lack of technology...

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Robo1 Binds to Heparin at Two Distinct Binding Sites

A team of Research Resource investigators, including members of the labs of Joshua Sharp and Kelley Moremen, have worked together to help determine the role of glycosaminoglycans (GAGs) in nervous system development. GAGs bridge a complex of two proteins, Robo1 and Slit2, to help...

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Glycosaminoglycan Builder

Glycosaminoglycan builder provides a point-and-click interface to build custom structures for all classes of glycosaminoglycans (GAGs). The web-tool is a simple interface where the user first chooses the desired class of GAG molecule and then enters the primary sequence by clicking through the set of...

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Slit Robo Interactions

Co-receptor function of heparan sulfate in Slit3-Robo4 signaling: Heparan sulfate, carried by the heparan sulfate proteoglycans, such as syndecan, binds Slit3 on wildtype endothelial cell surface to form the heparan sulfate-Slit3-Robo4 ternary complexes facilitating Slit3-Robo4 signaling. Genetic ablation of N-deacetylase-N-sulfotransferase-1 (Ndst1), an dual function...

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Sequencing of Complex Mixtures of Heparan Sulfate Tetrasaccharides by Chemical Derivatization and LC-MS/MS

Through a collaboration between synthetic chemists investigating chemical synthesis of defined heparan sulfate oligosaccharides (Chengli Zong, Andre Venot, and Geert-Jan Boons), analytical chemists developing a derivatization, separation, and MS/MS methods for separating and sequencing complex mixtures of heparan sulfate oligosaccharides (Rongrong Huang, Dandan Zhou, and...

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Dissemination, Contributions, & Achievements

Resource investigators actively disseminate our technologies through presentations, publication in journals and news articles. Representation at International Meetings Numerous presentations at International Symposium on Glycoconjugates, Proteoglycan and Glycobiology Gordon conferences and the Society for Glycobiology meetings. More than 50 presentations annually credit resource technologies, and investigators have...

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