Stephen Dalton, Senior Investigator
Richard Steet, Senior Investigator
This program provides various cells for use in developing the technologies, methodologies, and software that drive the various Technology Research and Development projects in our Resource. Each batch of each cell type is analyzed by several types of assays before it is use for other studies in order to assure standardization and quality control. Sufficient quantities of each cell type are subjected to study by our other programs in order that adequate statistical confidence in the results are achieved. Human embryonic stem cells that have been differentiated to various differentiated states have been provided by the program. Some of these, along with various markers used to define a particular cell type, are shown in the figure below. In addition, fibroblasts from patients with various Congenital Disorders of Glycosylation have been reprogrammed to iPSC (induced pluripotent stem cells) and differentiated to some of the cell types depicted in order to allow investigators to study the effects of mutations on particular cellular phenotypes.